Journal: International Journal of Molecular Sciences

Article Title: Diversity, Complexity, and Specificity of Bacterial Lipopolysaccharide (LPS) Structures Impacting Their Detection and Quantification †

doi: 10.3390/ijms25073927

Figure Lengend Snippet: SDS-PAGE analysis of the different LPSs used in the present study. 1— Y. pestis ; 2— Y. enterocolitica ; 3— B. pertussis 1414; 4— B. pertussis A100; 5— E. coli 0119; 6— E. coli J5; 7— N. meningitidis ; 8— P. aeruginosa P3; 9— S. Typhimurium Ra; 10— R. pickettii ; 11—LPS-MW Standard. Staining was performed according to Tsai and Frash . Rough-type LPSs migrate in the low-masses level at about 4 kDa.

Article Snippet: E. coli J5 LPS was extracted from strain ATCC 43745.

Techniques: SDS Page, Staining

Journal: International Journal of Molecular Sciences

Article Title: Diversity, Complexity, and Specificity of Bacterial Lipopolysaccharide (LPS) Structures Impacting Their Detection and Quantification †

doi: 10.3390/ijms25073927

Figure Lengend Snippet: MALDI mass spectra obtained for tetra-acyl E. coli J5 lipid A ( A ) and hexa-acyl E. coli J5 lipid A ( B ). Corresponding lipid A structures ( C ). Comparison of LAL and HEK-blue hTLR-4 activities ( D ). Fatty acids other than 14:0-3-OH are presented with different colors.

Article Snippet: E. coli J5 LPS was extracted from strain ATCC 43745.

Techniques: Comparison

Journal: PLoS ONE

Article Title: Effect of Solar Particle Event Radiation and Hindlimb Suspension on Gastrointestinal Tract Bacterial Translocation and Immune Activation

doi: 10.1371/journal.pone.0044329

Figure Lengend Snippet: Terminal ileum obtained 4 days post irradiation and/or 6 days post hindlimb suspension or from control animals was stained for LPS using a mouse mAb specific for E. coli LPS. A) Ileum from a control treated mouse. B) Ileum from a mouse irradiated with 2 Gy of 70 MeV protons. C) Ileum from a mouse subjected to hindlimb suspension. D) Ileum from a mouse treated with hindlimb suspension and irradiated with 2 Gy of protons. Original magnifications 200×. Tissue from 10 animals was analyzed for each condition.

Article Snippet: Tissue was cut into 6 μm sections in a Leica CM1850 cryostat, fixed in acetone (Thermo Fisher), and stained with rabbit anti-mouse Claudin-3 IgG (Thermo Fisher), goat anti-Lipopolysaccharide IgG (US Biologicals), or a murine monoclonal antibody to Escherichia coli (J5) LPS (Acris Antibodies) and detected with biotinylated anti-rabbit, goat, or mouse IgG (Sigma), streptavidin horseradish-peroxidase (Vector Labs), and AEC substrate (Sigma).

Techniques: Irradiation, Suspension, Control, Staining

Journal: PLoS ONE

Article Title: Repurposing a microfluidic formulation device for automated DNA construction

doi: 10.1371/journal.pone.0242157

Figure Lengend Snippet: User requests are sent from the TeselaGen DESIGN module to j5 to generate a scar-less combinatorial Golden Gate DNA assembly protocol. The automation module allocates resources, and initiates construction. Gel-purified, BsaI and DpnI-digested PCR products are generated off-chip according to the protocol, and then supplied to and then assembled together on the microfluidic chip. Assembled DNA (plasmids) are transformed into a host system of interest ( E . coli ) off-chip. The assembled DNA can then be sequence validated, and resulting reports generated.

Article Snippet: The TeselaGen software then calls j5 [ ] to generate the Golden Gate DNA assembly protocol [ – , , ] DNA parts were prepared following the j5-designed protocol.

Techniques: Purification, Generated, Transformation Assay, Sequencing

Journal: Theranostics

Article Title: Nanozyme-based colorimetric biosensor with a systemic quantification algorithm for noninvasive glucose monitoring

doi: 10.7150/thno.72152

Figure Lengend Snippet: Summary of color spaces, image acquisition, and processing for colorimetric glucose analysis using various body fluids

Article Snippet: CIELAB, SRGB , Glucose solutions real samples , iPhone 5S, Samsung J5, Scanner (Canon MF 4780dn) , JPEG , C-Measure Lite, Color Grab, Digital Color meter , .

Techniques: Software, Clinical Proteomics